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Becton Dickinson
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OZ Biosciences
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Merck KGaA
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Advisains
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Figure Lengend Snippet: DCFDA assay for the detection of intracellular ROS produced in response to oxidative stress caused by DW, L, [M(HN 3 O 2 )] 2+ (M(L); M = Mn, Fe, Co, and Cu), vehicle, and tert -butylhydroperoxide (TBHP) in (a) HCT116 and (b) CCD-986Sk cells. Cells were treated with 50 μM of metal complexes for 24 h and with 50 μM of TBHP alone for 4 h as the positive control. Data are shown as mean fold change (FC) to control ± standard error of the mean (SEM). The statistical analysis was performed using one way ANOVA-Dunnett's test and t test. Statistical significance is shown as: * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ns = nonsignificant as compared to control ( n = 3).
Article Snippet: ROS were detected using
Techniques: Produced, Positive Control